Azospirillum Based Bio Fertilizer assimilates atmospheric nitrogen and fixes the same in plants thus saving nitrogenius fertilizer application. The Bio Fertilizer contains azospirillum sp, a free living heterotrophic nitrogen fixing bacteria. The Bio Fertilizer secretes growth promoting substances in the plant root region facilitating quicker and vigorous growth of root and shoot. The Bio Fertilizer is suitable for various crops like paddy, wheat, maize, sugarcane, vegetables, flower crops, orchards and plantation crops. Azospirillum Based Bio Fertilizer can be applied to crops in combination with organic manure.