Bio-Fertilizer in Powder Form is suitable for various crops like bajra, sorghum, cotton, fodder crops and sugarcane responded favourably to azospirillum inoculation. The Bio-Fertilizer contains azospirillum that is an associative symbiotic nitrogen fixing bacterium, having higher nitrogen fixing potential. The Bio-Fertilizer is available in powder form and increases the grain yield by more than 25 percent. Bio-Fertilizer in Powder Form is applied through seed treatment and soil application, for direct sown crops.